The "Don't Tread on Me" flag gained fame amongst revolutionaries as a symbol of defiance towards British oppression

https://mylespgzl078.cavandoragh.org/final-honors-the-flag-s-significance-at-military-funerals

The "Don't Tread on Me" flag received status amongst revolutionaries as a image of defiance against British oppression, reflecting a spirit of resilience among colonists.